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专利名称：Purification methods for oligonucleotides
and their analogs
发明人：Jack T Johansen申请号：US10508799申请日：20030319
公开号：US20060035224A1公开日：20060216
摘要：The present invention discloses methods for separating oligonucleotides fromimpurities. In the methods of the invention, a target oligonucleotide, in a mixturecomprising the target oligonucleotide and an impurity, is separated from the impurityusing a titratable anion exchange composition. The target oligonucleotide is bound tothe titratable anion exchange composition and an eluting solution which increases in pHover time is passed through the titratable anion exchange composition with the targetoligonucleotide bound thereon. Preferably, the eluting solution does not substantiallyincrease its salt concentration. The target oligonucleotide is eluted and therebyseparated from the impurity which either elutes at a lower pH or a higher pH than thetarget oligonucleotide.
申请人：Jack T Johansen
地址：Concord MA US
国籍：US
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